Non-deuterated 1H NMR Spectrometric Method for the Rapid and Direct Analysis of Alcohol Content in Hand Sanitizers

In this study, a 1H nuclear magnetic resonance (NMR) method to quantify the alcohol content of alcohol-based hand sanitizers was developed and validated in non-deuterated solvent unlocked mode conditions. Deionized water used prepare solutions for no-D method, which also matched matrix samples. The generated integration areas were ethanol isopropanol sanitizer presented well-correlated calibration curves with detection limit 1.41% (v/v) 2.61% ethanol, respectively. experimental conditions compared results using D2O not significantly different (p > 0.05). Although there is no additional precautionary measure when handling D2O, solvents are more easily accessible, makes NMR-based analysis cost-effective.